Porcine Toxoplasmosis IgG Antibody ELISATest Kit

Porcine Toxoplasmosis IgG Antibody ELISA test kit

Catalog No. LSY-30001

1. Usage

Porcine Toxoplasmosis IgG antibody test kit is used for the detection of the Toxoplasmosis antibodies in the serum.

2. Principle

The test kit is enzyme immunoassay for the detection toxoplasmosis antibodies in porcine serum. Toxoplasmosis antigens are coated on 96-well plate, add sample into wells and then antibodies bound specifically by antigens, the unbound antibodies and other components will be removed by washing. Next add enzyme conjugation to specifically bind with the compound of antibodies and antigens on the microplate. The unbound enzyme conjugation will be removed by washing.Add the TMB substrate in the well, react to a blue product. At last, end the reaction by adding stop solution.

3. Reagents of the Test Kit

1Antigen coated microplate....................................... 2 pieces (96 wells each)

2TOX-IgGNegative control serum (green lid)................................ 1.5 mL/tube

TOX-IgGPositive control serum (red lid)..................................... 1.5 mL/tube

3Enzyme conjugate (yellow lid)..................................... 1 bottle (22 mL/bottle)

420×concentrated washing solution (white lid)............... 1 bottle (50 mL/bottle)

5Substrate A (purple lid)/B (yellow lid)................... 1 bottle each (12 mL/bottle)

6Stop solution (blue lid)............................................... 1 bottle (12 mL/bottle)

7Sample diluent solution (transparent lid)...................... 1 bottle (25 mL/bottle)

8Adhesive Foil................................................................................. 6 pieces

9Instruction....................................................................................... 1 piece

4. Material Required not Provided

1Microplate Reader(single-wave length: 450nm or double-wave length: 450/630 nm).

2Microplate Washer.

3Micropipettes, adjustable.

4Constant temperature box or incubator.

5Oscillator.