Aminohydantion (AHD) ELISA Test Kit
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of
Aminohydantion (AHD) in the sample. The coupling antigens are pre-coated on the micro-well
stripes. The Aminohydantion (AHD) in the sample and the coupling antigens pre-coated on the
micro-well stripes compete for the anti-AHD antibodies. After the addition of the enzyme conjugate,
the TMB substrate is added for coloration. The optical density (OD) value of the sample has a
negative correlation with the AHD in it. This value is compared to the standard curve and the AHD
concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.05 ppb
Detection limit
Tissue and honey················································································· 0.1 ppb
Shrimp and fish (some interference in shrimp and fish)·····················0.15 ppb
Recovery rate
Shrimp and fish··················································································· 90±15
Honey, meat, liver··············································································· 80±15%
Cross-reaction rate
AHD········································································································· 100%
AMOZ··································································································· ﹤0.1%
AOZ······································································································ ﹤0.1%
SEM······································································································﹤0.1%
3. Components
1) Micro-well strips: 12 strips with 8 removable wells each
2) 6× standard solution (1 mL each): 0 ppb, 0.05 ppb, 0.15ppb, 0.45 ppb, 1.35 ppb and 4.05 ppb
3) Enzyme conjugate (12 mL)·································································· red cap
4) Antibody working solution (7 mL)························································blue cap
5) Substrate A solution (7 mL)······························································· white cap
6) Substrate B solution (7 mL)·······························································black cap
7) Stop solution (7 mL)········································································ yellow cap
8) 20× concentrated washing buffer (40 mL)········································ white cap
9) 2× concentrated redissolving solution (50 mL)······················· transparent cap
10) 2-Nitrobenzaldehyde (10 mL)··························································· white cap